Abstract

Abstract

Marichyadi Taila is a polyherbal Ayurvedic oil formulation traditionally prescribed for various dermatological disorders (Kustha roga), many of which have microbial etiology. The rising concern over antimicrobial resistance (AMR) necessitates the scientific validation of traditional medicines. This study aimed to evaluate the in-vitro antibacterial and antifungal efficacy of three different batches (3698, 3718, and 3736) of Marichyadi Taila against common pathogenic microorganisms: Escherichia coli (Gram-negative bacteria), Staphylococcus aureus (Gram-positive bacteria), and Candida albicans (fungus). The antimicrobial activity was assessed using the Cup Plate Agar Diffusion method, comparing the zones of inhibition (ZOI) against standard control drugs (Gentamycin for bacteria and Fluconazole for fungi). The results indicated that Marichyadi Taila possesses moderate, dose-dependent antimicrobial activity against all tested strains. The maximum inhibitory efficiency was observed against C. albicans, with Batch No. 3718 showing the highest antifungal efficiency at 62.50% compared to Fluconazole. Against bacterial strains, the oil demonstrated greater efficacy against the Gram-positive S. aureus (up to 38.46% efficiency) than against the Gram-negative E. coli (up to 33.33% efficiency). Although its potency was significantly lower than the synthetic standard drugs, the consistent activity across all batches confirms the inherent broad-spectrum antimicrobial properties of Marichyadi Taila, supporting its traditional use in the management of infectious skin conditions.